Gene editing the phytoene desaturase alleles of Cavendish banana using CRISPR/Cas9
نویسندگان
چکیده
منابع مشابه
Efficient CRISPR/Cas9 Genome Editing of Phytoene desaturase in Cassava
CRISPR/Cas9 has become a powerful genome-editing tool for introducing genetic changes into crop species. In order to develop capacity for CRISPR/Cas9 technology in the tropical staple cassava (Manihot esculenta), the Phytoene desaturase (MePDS) gene was targeted in two cultivars using constructs carrying gRNAs targeting two sequences within MePDS exon 13. After Agrobacterium-mediated delivery o...
متن کاملPhytoene desaturase from Arabidopsis.
Carotenoids are isoprenyl pigments necessary for protection of the photosynthetic apparatus in a11 green plants. One of the intermediate steps in carotenoid biosynthesis is the conversion of phytoene to {-carotene via two dehydrogenation reactions catalyzed by the enzyme PDS. We have previously cloned cDNAs for this enzyme from soybean (Glycine max; Bartley et al., 1991) and tomato (Lycopersico...
متن کاملTomato and Tobacco Phytoene Desaturase Gene Silencing by Virus-Induced Gene Silencing (VIGS) Technique
Background and Aims: Virus-Induced Gene Silencing (VIGS) is a virus vector technology that exploits antiviral defense mechanism. By infecting plants with recombinant viruses containing host genes inserted in the viral genome, VIGS achieves the RNA silencing process. The purpose of this study was to investigate the possibility of tomato (Lycopersicon esculentum Mill.) and tobacco (Nicotiana be...
متن کاملBleaching herbicide flurtamone interferes with phytoene desaturase.
The mode of action of the furanone herbicide flurtamone and derivatives was investigated with cress seedlings and with the unicellular cyanobacterium Anacystis. Either in the light or in the dark these compounds inhibited the formation of alpha- and beta-carotene and all of the xanthophylls in the seedlings. Instead, phytoene, a precursor of colored carotenoids, was accumulated. In illuminated ...
متن کاملSome Physical Properties of Full-Ripe Banana Fruit (Cavendish variety)
Physical properties of fruits and vegetables are the subject of many researches because of its importance in designing of agricultural machinery. Banana fruit is one of important fruit. In this study some physical properties of banana fruit (Cavendish variety) were determined. Properties which were measured included weight of whole fruit peel and pulp weight, dimensions, surface area and projec...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Transgenic Research
سال: 2018
ISSN: 0962-8819,1573-9368
DOI: 10.1007/s11248-018-0083-0